plex 305 41390 lentiviral backbone vector Search Results


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Image Search Results


Journal: Cell reports

Article Title: Time-resolved proximity labeling of protein networks associated with ligand-activated EGFR

doi: 10.1016/j.celrep.2022.110950

Figure Lengend Snippet:

Article Snippet: Gateway entry clones pDONR223-EGFR-WT (#81926) were purchased from Addgene. pLEX-305 (#41390) lentiviral backbone vector was engineered to substitute the hPKG (phosphoglycerate kinase) promoter with the CMV (human cytomegalovirus) promoter plus APEX2 tag in the C-terminus of the expression gene. pDONR223_EGFR_WT (#81926) were transferred to pLEX305_Cterm_APEX2 destination vector using Gateway LR Clonase II enzyme mix (Thermo Fisher) based on the provided protocol.

Techniques: Transduction, Recombinant, Mass Spectrometry, Sequencing, Software